C7910-01J

Creatine Kinase MB Isoenzyme II, Recombinant (CKMB) (Pichia)

Size

£938.00

SKU:: C7910-01J
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 10mM Tris-HCl, pH 6.8, 0.5mM DTT, 0.5mM EDTA, 50% glycerol. No preservative added.
Extra Details:: Recombinant full length Creatine Kinase MB Isoenzyme with aa sequence identical to native enzyme. CK-MB is a dimeric protein comprised of M and B subunits. The doublet band migrating approximately 47kD is not resolved on most gels. Purified in the enzymatically active form. Reacts with monoclonal antibodies specific to MB isoenzyme in ELISA. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Unit Definition: One unit equals 1 micromole creatine phosphate formed/minute. Enzyme Activity: ≥700u/mg at 37°C at 340nm Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for 6 months after receipt at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.