C-Reactive Protein (CRP, MGC88244, PTX 1, PTX1) (HRP)

Size

£704.00

SKU:: C7907-21K
Purity:: ≥ 95% Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: CRP was immunoaffinity purified from rat serum.
Format:: Supplied as a liquid in 50mM HEPES, pH 7.1, 0.1 M sodium chloride, 0.2% BSA, 0.01% Thimerosal. Labeled with horseradish peroxidase (HRP).
Extra Details:: Pentraxins family of proteins acquired the name from their ability to form pentameric (or decameric) structures formed by non-covalent interactions. C-reactive protein (CRP or PTX1; mature chain 206 aa; chromosome 1q21-23) nonglycosylated, ~24kD monomer and ~118kD pentamer) is a ubiquitous protein found in both vertebrates and invertebrates. Originally CRP was defined as a substance, observed in the plasma of patients with acute infections, that reacted with the C polysaccharide of the Pneumococcus. It is one of the plasma proteins that are called acute phase reactants because of a pronounced rise in concentration after tissue injury or inflammation. In the case of CRP the rise may be 1000-fold or more. CRP is composed of 5 identical, 21,500MW subunits. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver. Those cells produce CRP detectable on lymphocytes. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:5000-1:25,000 Western Blot: 1:50-1:500 Optimal dilutions to be determined by the researcher. Molar Ratio of Enzyme to Antibody: ~4:1 Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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