C-Reactive Protein (CRP, MGC88244, PTX 1, PTX1) (HRP)

Size

£672.00

SKU:: C7907-21E
Purity:: Purified by immunoaffinity chromatography. >95%. by RID, SDS-PAGE and IEP.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: C-Reactive Protein from human serum/fluids.
Format:: Supplied as a liquid in 50mM HEPES, pH 7.1, 0.1M sodium chloride, 0.2% BSA, 0.01% Thimerosal. Labeled with horseradish peroxidase (HRP).
Extra Details:: Pentraxins family of proteins acquired the name from their ability to form pentameric (or decameric) structures formed by non-covalent interactions. C-reactive protein (CRP or PTX1; mature chain 206aa; chromosome 1q21-23) nonglycosylated, ~24kD monomer and ~118kD pentamer) is a ubiquitous protein found in both vertebrates and invertebrates. Originally CRP was defined as a substance, observed in the plasma of patients with acute infections, that reacted with the C polysaccharide of the Pneumococcus. It is one of the plasma proteins that are called acute phase reactants because of a pronounced rise in concentration after tissue injury or inflammation; in the case of CRP the rise may be 1000-fold or more. CRP is composed of 5 identical, 21,500D molecular weight subunits. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver; those cells produce CRP detectable on lymphocytes. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:10,000 ELISA: 1:10,000-1:50,000 Optimal dilutions to be determined by the researcher. Antibody Ratio: ~4:1 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Conjugated to horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

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