C7906-72B
CRACC (SLAMF7, SLAM family member 7, 19A, CSI, CD2-like receptor-activating cytotoxic cells, CD2 subset 1, CD319, CS1, Membrane protein FOAP-12, Novel Ly9, Protein 19A, UNQ576/PRO1138)
Size
£812.00
- SKU:
- C7906-72B
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Ala22-Gly224 of mouse CRACC, expressed in CHO cells.
- Clone:
- 9H101
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- CRACC, also known as CSI (CD2 subset I), is a type I transmembrane protein belonging to the CD2 subset of the Ig superfamily. CRACC is expressed on most NK cells and subsets of CD8 + cells, CD4 + cells and B cells. CRACC may play a role in the activation and effector function of T cells and NK cells. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (Direct): 0.5-1ug/ml with the appropriate secondary reagents to detect mouse CRACC. The detection limit for rmCRACC is ~1ng/well. Western Blot: 2ug/ml. Lysates were prepared from mouse thymus extract in reducing sample buffer, resolved by SDS-PAGE (30ug total protein/lane), and transferred to an Immobilon-P membrane. Use of this antibody under non-reducing conditions is not recommended. The blot was developed with 2ug/ml of C7906-72B and chemiluminescent detection substrate. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice