C7904-82R

COX 2 (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS 2)

Size

£672.00

SKU:: C7904-82R
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to the C-terminal region of human COX-2
Format:: Supplied as a liquid in PBS, pH 7.2, 50% glycerol, 0.02% sodium azide.
Extra Details:: COX-2 has been cloned from a variety of species including human, rat, and sheep and has a molecular weight of 72kDa.1,2,3,4, COX-2 from these species are approximately 90% identical at the amino acid level, whereas the homology between COX-1 and COX-2 is only about 60%. Expression of COX-2 is regulated by growth factors, tumor promoters, bacterial endotoxin and cytokines, including IL-1 beta.5,6 COX-2 is present in human breast tumors but is not expressed in normal breast tissue.7 Applications: Suitable for use in Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:200 Immunofluorescence: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.