C7904-82K
COX 2 (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS 2) (FITC)
Size
£761.00
- SKU:
- C7904-82K
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to aa580-599, NASSSRSGLDDINPTVLLKE of human COX-2. Species sequence homology: bovine and canine.
- Clone:
- 6D319 (CX229)
- Format:
- Supplied as a liquid in PBS, pH 7.2, 4mg/ml BSA, 0.02% sodium azide, 50% glycerol. Labeled with fluorescein isothiocyanate (FITC).
- Extra Details:
- Cyclooxygenase catalyzes the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins: the conversion of arachidonic acid to prostaglandin H2. Recent discoveries of the induction of cyclooxygenase biosynthesis by a variety of stimuli such as phorbol esters, lipopolysaccharides, and cytokines led to the hypothesis that the inducible form of cyclooxygenase, COX-2, is responsible for the biosynthesis of prostaglandins under acute inflammatory conditions. COX-2 is a 72kD protein which has been cloned from a variety of species including human, mouse, rat, and sheep. Applications: Suitable for use in Flow Cytometry and Immunocytochemistry. Not recommended for Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1ug/10e6 cells Immunocytochemistry: 2-5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice