C7903-89
Corticotropin-Releasing Factor Receptor 1 (CRHR1, CRFR1)
Size
£825.00
- SKU:
- C7903-89
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Ser24-Val415 from human Corticotropin-Releasing Factor Receptor 1 expressed in NS0 cells.
- Clone:
- 7H27
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Corticotrophin-Releasing Hormone Receptor 1 (CRHR-1), also known as CRFR-1, is a 7TM protein that belongs to the G protein-coupled receptor family 2. CRHR-1 is expressed in the brain and adrenal gland where it mediates corticotropin-induced effects on anxiety, depression, and stress-associated pathologies. The immunogen for this antibody corresponds to one of the four alternately spliced forms and has a 29 amino acid deletion in the first cytoplasmic domain. Human CRHR-1 shares 97% amino acid sequence identity with mouse and rat CRHR-1. Applications: Suitable for use in Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 8-25ug/ml using immersion fixed paraffin-embedded sections of human placenta Flow Cytometry: 2.5ug labels 10e6 human CRHR-1 transfected Ba/F3 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice