Corticoliberin, Pro (Pro-Corticoliberin, Pro-CRF, Pro-CRH, Pro Corticotropin releasing hormone, Pro Corticotropin-releasing factor)

Size

£825.00

SKU:: C7903-80Z
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: E. coli-expressed recombinant human Pro-Corticoliberin, aa25-196.
Format:: Supplied as a lyophilized powder in PBS containing 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: Pro-Corticoliberin (also Pro-CRF and Pro-CRH) is a predicted 19kD member of the CRF/Urotensin I family of hormones. It is expressed by placental synctiotrophoblasts, zona fasciculata adrenal cells and paraventricular neurons, and is induced by glucocorticoids. Pro-Corticoliberin is the precursor to a mature, 41 amino acid amidated Corticoliberin that induces pituitary ACTH and subsequent cortisol production by the adrenal. Human Pro-Corticoliberin is 170aa in length (aa25-194). If unprocessed, it undergoes N-terminal amidation to generate a polypeptide 168aa in length (aa27-194). Secreted Pro-Corticoliberin is both bioactive and binds to 37kD Corticoliberin-PB. In the placenta, Pro-Corticoliberin is only occasionally cleaved to generate mature Corticoliberin (aa152-192); in the brain, it undergoes active internal proteolysis to generate multiple fragments, including the 5kD mature peptide. Over aa25-196, human Pro-Corticoliberin shares 78% aa identity with mouse pro-Corticoliberin. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml ELISA: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice