C7900-80
CoREST (REST corepressor 1, Protein CoREST, KIAA0071, RCOR, RCOR1)
Size
£978.00
- SKU:
- C7900-80
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- GST fusion protein corresponding to residues 109-293 of human CoREST.
- Format:
- Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
- Extra Details:
- Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.1-0.5ug/ml detects CoREST in RIPA lysates from A431, 3T3/A31, Jurkat, and Hela cells. Reported to be detected in RIPA lysates from HEK 293, L6, PC12, C6 cells and liver, muscle, heart and brain tissue. Immunoprecipitation: 2.5ug immunoprecipitates CoREST from 250ug of A431 RIPA lysate. Immunocytochemistry: Positive immunostaining for CoREST in cells fixed with 4% paraformaldehyde pH 7.3 for 10-15 minutes Immunohistochemistry: Reported to detect CoREST in tissue sections. Included Positive Control: A0001-50: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice