C7865-19A-ML650

Contactin-associated protein 1 (Caspr1) (MaxLight 650)

Size

£1,138.00

SKU:: C7865-19A-ML650
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to rat Contactin-associated protein 1. Species Sequence Homology: mouse, 100%.
Clone:: 11C235 (K65/35)
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Extra Details:: MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Contactin-associated protein 1(Caspr) is a single-pass type I membrane protein belonging to the neurexin family and is comprised of one F5/8 type C domain, one fibrinogen C-terminal domain, two EGF-like domains, and four laminin G-like domains. Caspr is well documented for its interaction with contactin, an interaction that is crucial to the transport of Caspr to the plasma membrane, the mylination of glial cells, and the generation of axoglial junctions. Studies also suggest that Caspr may play a regulatory role in the cell surface expression of contactin and its targeting to different axonal domains. Expression of Caspr is largely observed in the brain, mainly in CNS myelinated nerve fibers within paranodal axoglial junctions. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Paraffin): Heat-induced epitope retrieval in citrate buffer, pH 6.0 is required. Optimal dilutions to be determined by the researcher. Positive Control: Rat brain membrane tissue lysate Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

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