Connexin 45 (GJC1, Gap Junction protein, gamma 1, 45kD, Cx45, CX45, DKFZp686P0738, Gap junction alpha-7 protein, Gap junction gamma-1 protein, GJA7)

Size

£754.00

SKU:: C7858-05D
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 14aa near the N-terminal of mouse Connexin 45 conjugated to KLH. Species Sequence Homology: chicken, canine, hamster, guinea pig, rat and human.
Format:: Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Extra Details:: Gap junctions are composed of transmembrane channels that link the cytoplasm of neighboring cells. They differ from other membrane channels since they exist between two cells. Gap junctions are relatively non-specific and allow passive diffusion of small molecules up to 1000 Dalton. The junctions exist in almost all vertebrate and non-vertebrates cells. It is believed that gap junction play an important for intercellular communications and affect growth and differentiation of cells. Gap junctional channel is composed of a hemichannel (connexon) in the cell membrane of one cell joined in mirror symmetry with a connexon in the opposing cell. Each connexon is an oligomer of six protein subunits that define the axial aqueous pore. Molecular cloning studies have identified a family of at least 12 highly related Connexins that are designated according to MW, Cx26-50. Hydropathy analyses of Cx sequences predicts 4 transmembrane (TM), 2 extracellular (EC), and 3 cytoplasmic (CP) domains. The EC, TM, and N-terminal CP domains are well conserved among family members, while central and C-terminal domains are highly variable in both sequence and size. The N and C-termini are predicted to be cytoplasmic. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilutions: ELISA: 1:100,000 using 50-100ng control peptide/well. Optimal dilutions to be determined by the researcher. Recommended Control Peptide: C7858-05E: Connexin 45, Mouse (CX45, Gap Junction Alpha 6 Protein, CXA-6) (Control Peptide) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice