C7857-BIOTIN

Connexin 43 (Cx43, Gap Junction Alpha 1 Protein, CxA-1) (Biotin)

Size

£1,081.00

SKU:: C7857-BIOTIN
Purity:: Purified by Protein A affinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to aa252-270 at the C-terminal of the mouse connexin 43 sequence. Species Sequence Homology: bovine
Clone:: 2Q250 (4E6.2)
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Extra Details:: Mouse connexin 43 is a 382 amino acid gap junction protein with a predicted MW ~43kD. It is prominently expressed in heart. Gap junctions are composed of transmembrane channels that link the cytoplasm of neighboring cells. They differ from other membrane channels since they exist between two cells. Gap junctions are relatively non-specific and allow passive diffusion of small molecules up to 1000 Daltons. The junctions exist in almost all vertebrate and non-vertebrate cells. It is believed that gap junction play an important for intercellular communications and affect growth and differentiation of cells. Gap junctional channel is composed of a hemichannel (connexon) in the cell membrane of one cell joined in mirror symmetry with a connexon in the opposing cell. Each connexon is an oligomer of six protein subunits that define the axial aqueous pore. Molecular cloning studies have identified a family of at least 12 highly related Connexins that are designated according to mol. wt, Cx26-50. Hydropathy analyses of Cx sequences predicts 4 transmembrane, 2 extracellular (EC), and 3 cytoplasmic (CP) domains. The EC, TM, and N-terminal CP domains are well conserved among family members, while Central and C-terminal domains are highly variable in both sequence and size. The N and C-termini are predicted to be cytoplasmic. Applications: Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and ELISA. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Frozen): Using keratinocytes of the middle and upper epidermal layers of frozen human skin tissue and mouse keratinocytes and rat heart Optimal dilutions to be determined by the researcher. Control Peptide: C7857-04 Positive Control: Mouse brain tissue lysate Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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