Connexin 43, phosphorylated (Ser368) (Connexin-43, ]Cx43, Cx43alpha1, ADFNB38, Gap Junction alpha-1 Protein, Gap Junction 43kD Heart Protein, GJA1, Gap Junction Protein alpha-like, GJAL, HSS, Oculodentodigital Dysplasia, ODDD, Syndactyly Type III, SDTY3)

Size

£696.00

SKU:: C7857-03P
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser368 from rat Connexin43, conjugated to KLH. Species Sequence Homology: canine, chicken, human, guinea pig, bovine, human and zebrafish
Format:: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Extra Details:: Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy. Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of Connexin43 is known to be regulated by phosphorylation at Ser368 by protein kinase C. Phosphorylation of Ser368 by PKC induces a conformational change of Connexin43 that results in a decrease in gap junction permeability. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.