Connexin 40 (CX40, Gap Junction Alpha 5 Protein, CXA-5)

Size

£825.00

SKU:: C7856
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide consisting of 19aa within the C-terminal cytoplasmic domain of mouse Cx40. Species Sequence Homology: human and rat Cx40 (100%), chicken Cx42 (77%), human Cx50 (87%), rat Cx50 (81%) and sheep Cx49 (87%).
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 15mM sodium chloride, 0.05% sodium azide.
Extra Details:: Mouse Connexin 40 is a 358aa gap junction protein with a predicted M.W. of ~40kD. It is prominently expressed in lung, heart and skin (see reviews: Kumar & Giula 1996; White, 1995; Evans, 1994; Beyer, 1990; Henneman, 1992; Traub, 1994; Goliger & Paul 1994). One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. A connexon is composed of a hexamer of connexins. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000 detected Connexin 40 in 10ug of Jurkat lysates. Immunohistochemistry: 1:50 detected Connexin 40 immunoreactivity associated with the gap junction between the cardiac myoepithial cells in mouse heart tissue sections. Optimal dilutions to be determined by the researcher. Control Peptide: C7856-01: Connexin 40, Mouse, Control Peptide (CX40, Gap Junction Alpha 5 Protein, CXA-5) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice