Connexin 40 (CX40, Gap Junction alpha 5 Protein, CXA-5) (Not for Export EU)

Size

£704.00

SKU:: C7856-02
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A 19 amino acid peptide sequence within the C-terminal cytoplasmic domain of Mouse Cx40 (2) Peptide was coupled to KLH (carrier protein)
Format:: Supplied as a liquid, neat serum, 40% glycerol.
Extra Details:: Applications: Suitable for use in ELISA, Western Blotting, Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: We recommend testing of antiserum at 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using Chemiluminescence technique. An antibody made to a similar epitope has been shown to recognize 40kD band in various tissues (3). ELISA (1:10K-100K using 50-100ng Cx40 control peptide/well. Immunoprecipitations. We recommend the use of affinity pure antibodies at 2-10ug affinity pure antibodies (3). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.