C7854-02-BIOTIN

Connexin 32 (CX32, C’B1, Gap Junction Beta1 Protein, Gap Junction Liver Protein) (Biotin)

Size

£1,070.00

SKU:: C7854-02-BIOTIN
Purity:: Purified IgG
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Directed against an epitope located between residues 95-125 in the central cytoplasmic loop of rat Cx32.
Clone:: 2Q252 (M12.13)
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Extra Details:: Applications: Suitable for use in Immunohistochemistry (lightly formaldehyde fixed tissues) and Western Blot. Other applications have not been tested. Recommended Dilution: Western blot: Detects a 27kD Connexin 32 polypeptide in blots of rat hepatocyte gap junctions and an additional 47kD band corresponding to aggregates. Optimal dilution to be determined by the researcher. Hybridoma: Produced by the immunization of BALB/c mice with isdolated rat junctional complexes. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.