C7853-61A
Connexin 31.1 (CX31.1.1, Gap Junction Beta 5 Protein, CXB-5) (Not for Export EU)
Size
£704.00
- SKU:
- C7853-61A
- Purity:
- Serum
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- A 14 amino acid peptide sequence within the 3rd cytoplasmic domain of the rat Cx31.1 (2) was used to generate anti-rat Cx31.1. Amino terminal Cysteine was added for conjugation to KLH (carrier protein)
- Format:
- Supplied as a liquid, neat serum, 40% glycerol.
- Extra Details:
- Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. An antibody raised against this epitope has been used for westerns (3). ELISA (1:100K using 50-100ng CX31.1 control peptide/well. Immunoprecipitations. We recommend the use of affinity pure antibodies at 2-10ug affinity pure antibodies. An antibody made to a similar epitope has been used for Immunoprecipitations (3). A similar protocol is recommended for this antibody. Immunohistochemistry & Immunofluorescence: we recommend the use of affinity purified antibody at 2-20ug/ml in formaldehyde fixed tissue (3). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice