Connexin 31 (CX31, Gap Junction Beta 3 Protein, CXB-3) (Not for Export EU)

Size

£704.00

SKU:: C7853-05B
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A 17 amino acid peptide sequence within the 1st cytoplasmic domain of the rat Cx31 (2) was used to generate anti-Mouse Cx31. Amino terminal Cysteine was added for conjugation to KLH (carrier protein)
Format:: Supplied as a liquid, neat serum, 40% glycerol.
Extra Details:: Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. An antibody raised against a GST-fusion has been shown to recognize ~31kD band in skin (3). ELISA (1:100K using 50-100ng CX31 control peptide/well. Immunohistochemistry & Immunofluorescence: we recommend the use of affinity purified antibody at 2-20ug/ml in formaldehyde fixed tissue (3). Optimal dilutions to be determined by the researcher. Control Peptide: C7853-61D Connexin 31, Mouse, Control Peptide (CX31, Gap Junction Beta 3 Protein, CXB-3) Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.