C7851-03
Complexin 2 (CPX II, Synaphin-1)
Size
£787.00
- SKU:
- C7851-03
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Purified, E. coli-derived, recombinant human Complexin-2 (rhCPX II; aa 1-134; UniProt # Q6PUV4).
- Format:
- Supplied as lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- Complexin-2 (CPX II; also known as synaphin-1) is a 19kD, cytosolic protein that belongs to the complexin/synaphin family of molecules. It is expressed in excitatory neurons and serves as a block against premature granule release by its binding to the cis-SNARE complex that contains SNAP25, VAMP2 and STX1A. Human Complexin-2 is 134 amino acids (aa) in length and contains one coiled-coil domain (aa 28-84) that incorporates part of a SNARE complex interaction sequence (aa 41-97). There is one potential splice variant that shows a premature truncation after Glu114. Human Complexin-2 is identical in aa sequence to that for mouse and rat Complexin-2. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml. Lysates from human, mouse and rat brain tissue were resolved by SDS-PAGE, transferred to Immobilon-P membrane and immunoblotted with 1ug/ml goat anti-Complexin-2. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice