Complement Factor B (CFB, BF, BFD, C3/C5 Convertase, CFAB, Glycine-rich beta Glycoprotein, GBG, FB, PBF2, Properdin Factor B) (Not for Export EU)

Size

£466.00

SKU:: C7850-60L
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Human Properdin Factor B
Format:: Supplied as a lyophilized powder. Preservative free. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Extra Details:: Properdin factor B is a heat-labile serum protein active in the alternative complement activation pathway. It is binding to the C3b component of complement. The protein has a molecular weight of 92000 and its mean concentration in human plasma is 200ug/ml. Applications: Suitable for use in ELISA, Immunoelectrophoresis and Double Radial Immunodiffusion. Other applications not tested. Recommended Dilution: Immunoelectrophoresis: 120ul against 2ul human serum. Double Radial Immunodiffusion: Use a rosette arrangement with 10ul in a 3mm diameter center well and 10ul in 3mm diameter peripheral wells. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.