C7850-23

Complement C5, Human

Size

£800.00

SKU:: C7850-23
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human complement component C5.
Clone:: 5F207
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
Extra Details:: C5 component of human complement is a plasma protein consisting of two polypeptide chains, a, b, bound by disulphide bonds. It has a molecular weight of 190000. The activated protein forms with the complement factors C6-C9 the membrane attack complex. The average concentration in plasma is about 70mg/ml. Applications: Suitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Frozen): 1:10; Incubation Time 1hr at RT. Optimal dilution to be determined by researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.