Complement Component 4 Binding Protein (C4 Binding Protein, C4BP, Complement Component 4 Binding Protein alpha Chain, C4b Binding Protein alpha Chain, C4BPA, Complement Component 4 Binding Protein beta Chain, C4b Binding Protein beta Chain, C4BPB, Proline-rich Protein, PRP) (Not for Export EU)

Size

£479.00

SKU:: C7850-19E
Purity:: Serum.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Human C4 binding protein.
Format:: Supplied as a lyophilized powder. Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Extra Details:: C4-binding protein is a plasma protein with a molecular weight of 500000 and consists of 6 chains. Its concentration in serum is about 250ug/ml. It is one of the inhibitors of the complement activation system. The protein combines up to six molecules of C4b at or near the C2 combining site and is thus preventing further association with C2. Applications: Suitable for use in ELISA, Immunoelectrophoresis and Radial Immunodiffusion. Other applications not tested. Recommended Dilutions: Immunoelectrophoresis: 120ul against 2ul human plasma or equivalent in agarose-plates. Double Radial Immunodiffusion: Use a rosette arrangement with 10ul in 3mm diameter center well and 2ul plasma samples (neat and serially diluted) in 2mm diameter peripheral wells. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.