C7850-18J-HRP
Complement Component 4d (Complement C4d, C4 Complement C4d Region, C4d, C4d Fragment) (HRP)
Size
£1,081.00
- SKU:
- C7850-18J-HRP
- Purity:
- Purified by Protein A affinity chromatography from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified human Complement Component 4d.
- Clone:
- 10B397 (057-51.5.1.6)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Horseradish peroxidase (HRP).
- Extra Details:
- The complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4b’s alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonisation and activation of the classical pathway. This requires C4 binding protein or CR1 as a cofactor. iC4b is further degraded into C4d and C4c. The short half life of C4d means that it is present in serum at high enough concentrations to make it a useful marker for classical complement activation. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Positive Control for Immunohistochemistry: Kidney fro post streptoccal glomerulonephritis patients Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice