C7850-18J-APC

Complement Component 4d (Complement C4d, C4 Complement C4d Region, C4d, C4d Fragment) (APC)

Size

£1,081.00

SKU:: C7850-18J-APC
Purity:: Purified by Protein A affinity chromatography from ascites.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified human Complement Component 4d.
Clone:: 10B397 (057-51.5.1.6)
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Extra Details:: The complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4b’s alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonisation and activation of the classical pathway. This requires C4 binding protein or CR1 as a cofactor. iC4b is further degraded into C4d and C4c. The short half life of C4d means that it is present in serum at high enough concentrations to make it a useful marker for classical complement activation. Applications: Suitable for use in FLISA and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Positive Control for Immunohistochemistry: Kidney fro post streptoccal glomerulonephritis patients Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Shipping Conditions:: Blue Ice