C7850-18B1-ML550
Complement Component 4c (Complement C4c, C4c) (MaxLight 550)
Size
£971.00
- SKU:
- C7850-18B1-ML550
- Purity:
- Purified by Protein A affinity chromatography from ascites.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified human Complement Component 4c.
- Clone:
- 10B395 (10-12)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
- Extra Details:
- MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. The complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4b’s alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonization and activation of the classical pathway. iC4b is further degraded into the inactive fragments C4d and C4c. C4c consists of two a chain fragments, alpha 4 (13kD) and alpha 3 (25kD) linked to a beta chain through disulphide bridges and a gamma chain. It is expressed in the blood plasma. Applications: Suitable for use in FLISA, Flow Cytometry, Immunohistochemistry, and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Histology Positive Control Tissue: Kidney sections from post-streptococcal glomerulonephritis patients Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice