C7850-18B1-BIOTIN
Complement Component 4c (Complement C4c, C4c) (Biotin)
Size
£971.00
- SKU:
- C7850-18B1-BIOTIN
- Purity:
- Purified by Protein A affinity chromatography from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified human Complement Component 4c.
- Clone:
- 10B395 (10-12)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
- Extra Details:
- The complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4b’s alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonization and activation of the classical pathway. iC4b is further degraded into the inactive fragments C4d and C4c. C4c consists of two a chain fragments, alpha 4 (13kD) and alpha 3 (25kD) linked to a beta chain through disulphide bridges and a gamma chain. It is expressed in the blood plasma. Applications: Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Histology Positive Control Tissue: Kidney sections from post-streptococcal glomerulonephritis patients Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice