C7850-16B
Complement C4
Size
£1,068.00
- SKU:
- C7850-16B
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Thymocytes decorated with Thy-1 antibody and complement components.
- Clone:
- 6A530 (16D2)
- Format:
- Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
- Extra Details:
- Murine complement factor C4, formerly known as Gg protein, consists of an alpha-, beta-, and gamma-chain. The classical pathway of complement and the Mannose binding lecting activation pathway converge at C4. C1s, MASP-1 and MASP-2 cleave C4 resulting in the formation of C4a and C4b. the latter can be cleaved to result in C4c and C4d in which step all functional sires are lost. The C7850-16B reacts with intact C4, C4b, C4d. C4 is an acute phase protein that is produced by hepatocytes, monocytes and intestinal epithelial cells. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:50 Western Blot: 1:50. Use non-reduced sample Immunoprecipitation: 1:50 mmunohistochemistry (Frozen): 1:300 Fix sections with 10% buffered formalin solution for 10 minutes @ RT. Immunofluorescence (Frozen): 1:50. Fix sections with ice-cold acetone and block with 2% BSA, FCS and PBS. Optimal dilutions to be determined by the researcher. Positive Control: T-cell regions of mouse spleen Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice