Complement C3, C3c (Complement Component C3c, C3/C3c, Acylation Stimulating Protein Cleavage Product, ASP, Complement Component 3, Complement Factor 3, CPAMD1) (HRP)

Size

£704.00

SKU:: C7850-14U4
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: C3c isolated and purified from pooled normal rat serum.
Format:: Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Labeled with Horseradish Peroxidase (HRP). Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Extra Details:: C3c is the major fragment resulting from C3 cleavage by C3 convertase and factor I. It is composed of an intact beta chain bound to two fragments of the alpha chain. Applications: Suitable for use in ELISA, Immunoelectrophoresis and Double Radial Immunodiffusion. Other applications not tested. Recommended Dilution: ELISA: 1:1000-1:5000 Optimal dilutions to be determined by the researcher. Peroxidase/IgG protein molar ratio (E/P): ~1.8 Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.