C7850-14C5
Complement C3c (FITC)
Size
£461.00
- SKU:
- C7850-14C5
- Application:
- FLISA
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Porcine
- Immunogen:
- C3c is isolated and purified from pooled normal human serum.
- Format:
- Supplied as a lyophilized powder. Preservative free. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2. Labeled with FITC.
- Extra Details:
- Human Complement component 3, C3 (alternative names include acylation-stimulating protein (ASP) C3 is encoded by gene located 19p13.3-p13.2. Because C3, C4, and C5 are strikingly similar suggesting a common evolutionary origin. C3 is an acute phase reactant. Synthesis of C3, a glycoprotein, is induced during acute inflammation. The liver is the main site of synthesis, although small amounts are also produced by activated monocytes and macrophages. A single chain precursor (pro-C3) of approximately 200kD is found intracellularly; the cDNA shows that it comprises 1,663aa. This is processed by proteolytic cleavage into alpha (~115kD) and beta subunits (~75kD) which in the mature protein are linked by disulfide bonds. Applications: Suitable for use in FLISA, Immunoelectrophoresis and single and double Radial Immunodiffusion (Mancini, Ouchterlony) to identify the presence of complement C3c or to determine its concentration. Other applications not tested. Recommended Dilution: FLISA: 1:20- 1:80. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice