C7850-13V-HRP

Complement C3b, inactivated (iC3b, Complement Component 3, C3, AHUS5, ARMD9, ASP, C3 and PZP-like alpha-2-macroglobulin Domain-containing Protein 1, CPAMD1) (HRP)

Size

£1,266.00

SKU:: C7850-13V-HRP
Purity:: Purified by Protein A affinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified human inactivated Complement C3b
Clone:: 10D230 (013III-1.16)
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Horseradish peroxidase (HRP).
Extra Details:: Cleavage of C3b into iC3b by the protease factor I, in the presence of cofactors, changes the structure and resulting binding properties, preventing binding by complement factor B and properdin. This prevents further progression of the complement pathway, providing another mechanism by which the complement pathway may be regulated. iC3b is thought to play a role in inducing tolerance, binding complement receptor type 3 on antigen presenting cells and stimulating them to produce transforming growth factor beta2 and interleukin-10. It is thought to stimulate B-cells via the CD21 receptor providing a link between the innate and adaptive immune responses. Additionally iC3b acts as an opsonin which is recognised by complement receptor 3 expressed on leukocytes or complement receptor of the immunoglobulin superfamily (CRIg) on Kupffer cells, stimulating phagocytosis of the tagged cells. iC3b levels are often elevated in diseases such as Systemic Lupus Erythematosis and Rheumatoid arthritis. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Frozen and paraffin sections Optimal dilutions to be determined by the researcher. Positive Control: Kidney from post streptoccal glomerulonephritis patients Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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