C7553
Collectrin (TMEM27, Transmembrane protein 27 Collectrin Precursor, NX17, UNQ679, PRO1312) (Biotin)
Size
£934.00
- SKU:
- C7553
- Application:
- WB
- Purity:
- Purified by Immunoaffinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to Glu15-Ile144 of mouse Collectrin extracellular domain, expressed in NSO cells. Species Sequence Homology: rat; 92%, human; 86%
- Format:
- Supplied as lyophilized powder from PBS, 7.4, 50ug BSA per 1ug antibody. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
- Extra Details:
- Collectrin (also Transmembrane protein 27) is a 37-43kD member of the MEM27 family of proteins. It is expressed on pancreatic beta-cells where it regulates insulin secretion, and on renal tubular epithelium where it mediates amino acid transport. Mature mouse Collectrin is a 208aa type I transmembrane glycoptotein. It contains a 127aa extracellular region (aa15-141) and a 60aa cytoplasmic domain (aa163-222). Collectrin dimerizes on the cell surface, and undergoes proteolytic cleavage to generate a 26kD soluble fragment. Over aa15-144, mouse Collectrin shares 92% and 86% aa identity with rat and human Collectrin, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice