C7510-98H
Collagen Type XXV, alpha 1 (COL25A1, CLAC)
Size
£780.00
- SKU:
- C7510-98H
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Gly189-Lys654 from human COL25A1 expressed in NS0 cells
- Clone:
- 11D6
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with sterile 200ul PBS.
- Extra Details:
- Collagen XXV a1 (COL25A1), also known as CLAC-P and AMY, is an 80kD member of the transmembrane (TM) collagen family. It is type II TM hydroxylated glycoprotein that is expressed by neurons. Proteolytic cleavage generates 70kD monomers that either dimerizIgG2Be or trimerize, and bind to fibrillar amyloid b-peptide. Human COL25A1 is 654aa in length. It has a 600aa extracellular domain (aa55-654) that contains seven collagen-like regions and is cleaved by furin between Arg112 and Glu113. Multiple splice forms may exist. One shows a 5aa substitution for (aa441-654), while a second shows a 2aa substitution for (aa616-654). Three other isoforms show splicing over aa165-245 accompanied by complex splicing at the C-terminus. Over (aa189-654), human COL25A1 is 95% aa identical to mouse COL25A1. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA (Direct): 0.5-1ug/ml Immunohistochemistry (Paraffin): 25ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Immunohistochemistry: Human Alzheimner’s brain tissue sections. Hybridoma: Myeloma cells with B cells from Balb/c mice. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice