Collagen XIII A1 (Col13a1, Collagen alpha-1(XIII) Chain)

Size

£843.00

SKU:: C7510-94B
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant protein corresponding to Glu107-Gln565 from mouse Collagen XIII A1, expressed in NS0 cell line.
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: Collagen XIII A1 is an 85-95kD protein in the type 2 transmembrane collagen family. Mature mouse Collagen XIII A1 consists of a 40aa cytoplasmic domain, a 19aa transmembrane segment, and a 692aa extracellular domain (ECD). The ECD contains three collagenous regions separated by shorter non-collagenous regions. Within comparable regions of the ECD, mouse Collagen XIII A1 shares 85% and 88% aa sequence identity with human and rat Collagen XIII A1, respectively. Mouse Collagen XIII A1 is extensively spliced, with some isoforms showing a tissue specific distribution. Collagen XIII A1 is widely expressed during development and in the adult. It localizes to intercellular adherens junctions and cell-matrix focal adhesions. Collagen XIII A1 assembles into disulfide-linked trimers, a process that is enhanced by proline hydroxylation. Trimerization involves triple helix formation within the collagenous domains, although portions of the non-collagenous regions can also form coiled coils. The ECD of trimeric Collagen XIII A1 is an extended rod-like structure with two flexible hinges that correspond to non-collagenous regions. Collagen XIII A1 clusters in cholesterol-rich domains on the plasma membrane, and it can be cleaved from the cell surface or intracellularly by a furin-like protease. Collagen XIII A1 binds the extracellular matrix molecules fibronectin, heparin, integrin A1 nidogen-2, perlecan. The shed ECD retains its ability to bind fibronectin and can interfere with matrix formation. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml detects Collagen XIII A1 in lysates of HT1080 human fibrosarcoma cell line Immunohistochemistry: 5-15ug/ml in immersion fixed frozen sections of mouse embryo (13 d.p.c.) Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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