C7510-94A
Collagen Type XIII (COL13A1, Collagen 13, alpha1)
Size
£837.00
- SKU:
- C7510-94A
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Recombinant corresponding to Glu109-Leu668 from human Collagen XII, expressed in CHO cells.
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- COL13A1 (collagen 13-alpha 1) is a 95 kDa member of the transmembrane group of the collagen family of proteins. It is a type II transmembrane glycoprotein that is expressed by multiple cell types, including fibroblasts, endothelial cells, and cardiac muscle. COL13A1 forms disulfide-linked homotrimers and participates in cell adhesion by binding to the integrin alpha 1 subunit, nidogen-2, and fibronectin. Human COL13A1 is 717 amino acids (aa) in length (SwissProt #:Q5TAT6). It has an N‑terminal 44 amino acid cytoplasmic region plus a 656 amino acid extracellular domain (ECD) (aa 62-717). The ECD contains four non-collagenous (NC) regions (aa 1‑121, 217-269, 442-463, and 700-717) interspersed among three collagenous (COL) domains (aa 122-216, 270-441, and 464-699). Multiple splice forms exist and typically involve deletions of 12-30 aa between aa 220-705. Proteolytic cleavage after Arg108 generates an 85-90 kDa soluble form. Over aa 109-668 (based on a human isoform [NP_542992} that shows a deletion of aa 239-260, 551-565, and 616-627), human COL13A1 shares 91% aa sequence identity with mouse COL13A1. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml detects COL13A1 in lysates of HUVEC human umbilical vein endothelial cells Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice