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C7510-61J

Collagen Type VI, X-adsorbed

Size

£576.00

SKU:
C7510-61J
Purity:
Purified by immunoaffinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Rabbit
Immunogen:
Collagen Type VI from human and bovine placenta
Format:
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide
Extra Details:
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:2000-1:4000. 1ug of Collagen VI was used in a standard sandwich ELISA with Goat anti-Rabbit IgG [H&L] (HRP conjugated) and (ABTS (2,2'-azino-bis- [3-ethylbenthiazoline-6- sulfonic acid]) as a substrate for 30 minutes at room temperature. Western Blot: 1:250-1:2500 Immunoprecipitation: 1:50 Immunohistochemistry (Frozen tissue sections): 1:25-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice