C7510-60J
Collagen Type VI
Size
£819.00
- SKU:
- C7510-60J
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified human Collagen Type VI
- Clone:
- 2Q637 (VI-26)
- Format:
- Supplied as a liquid in PBS, pH 7.0, 2% BSA (protease-free).
- Extra Details:
- The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha 1(VI), alpha 2(VI), and alpha 3(VI) chains. Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 10ug/ml Immunohistochemistry: 1-10ug/ml on acetone fixed paraffin-embedded tissues or frozen tissues. Not recommended for formalin fixed tissues. Epitope retrieval suggestions are treatment with 0.04% trypsin in 0.01M CaCl2, 0.05M Tris-HCl, pH 7.6, 37ºC for 10 min before the non specific peroxidase block. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice