Collagen Type IV (Arresten, ASLN, ATS, CA54, Canstatin, COL4A1, COL4A2, Collagen Alpha 1(IV) Chain, Collagen Alpha 2(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen of Basement Membrane Alpha 1 Chain, Collagen of Basement Membrane Alpha 2 Chain, Collagen Type IV Alpha 1, Collagen Type IV Alpha 2, DKFZp686I14213, FLJ22259, Goodpasture antigen) (HRP)

Size

£1,088.00

SKU:: C7510-51-HRP
Purity:: Purified by immunoaffinity chromotography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Native Collagen Type IV from mouse EHS tumor.
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Extra Details:: Type IV collagen is a major component of the basement membrane and it associates with laminin, entactin and heparin sulfate proteoglycans to form sheet-like structures that separates epithelium from connective tissue. It is a long triple helix containing several interruptions, which is characterized by a specific carboxylterminal, non-collagenous NC1 domain. It is composed of six genetically different alpha (IV) chains, alpha1 (IV) to alpha 6(IV) and their genes are paired on three different chromosomes in a head-to-head arrangement. The alpha 1 gene and the alpha 2 gene are localized on chromosome 13q34, the alpha 3 gene and the alpha 4 gene are on chromosome 2q36, and the alpha 5 gene and the alpha 6gene are on chromosome Xq22. Type IV collagen can be used as a thin coating on tissue culture surfaces to promote cell attachment and proliferation and to study its effects on cell behavior. Applications: Suitable for use in Immunofluorescence, Immunohistochemistry and ELISA. Other applications have not been tested. Recommended Dilution: Immunofluorescence: 1:40-1:160 Immunohistochemistry (paraffin): 1:250-1:1000 (Reccommended Immunohistochemistry fixatives: AFA, buffered formalin (5-10%) or Bouin’s) ELISA: 1:100-1:400 Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1903-30U: IgG, H&L, X-adsorbed (HRP) ShxRb Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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