Collagen Type IV (Arresten, ASLN, ATS, CA54, Canstatin, COL4A1, COL4A2, Collagen Alpha 1(IV) Chain, Collagen Alpha 2(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen of Basement Membrane Alpha 1 Chain, Collagen of Basement Membrane Alpha 2 Chain, Collagen Type IV Alpha 1, Collagen Type IV Alpha 2, Goodpasture antigen) (Not for Export EU)

Size

£854.00

SKU:: C7510-50C
Purity:: Serum.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Human placental type IV collagen.
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: As neoplastic cells of invasive carcinomas often lack a continuous basement membrane, the antiserum is useful to distinguish between non-invasive and invasive lesions. Additionally, it can be used for the differentiation of bullous lesions in dermatopathology. In immunohistochemistry no crossreactivity with other collagens at optimal dilutions. In immunoblotting, a slight crossreactivity with collagen type V is observed. Applications: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:50-1:100 Western Blot: 1:50-1:100 Immunohistochemistry (Frozen tissue and paraffin sections): 1:50-1:100. Proteolytic treatment required; enzyme: pepsin. Incubate 1 hour at RT or overnight at 4ºC. Optimal dilutions to be determined by the researcher. Recommended Positive Control: Skin and kidney Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.