Collagen Type III (Collagen alpha-1(III) Chain, Collagen III alpha 1 Chain Precursor, Collagen III alpha 1 Polypeptide, Collagen Type III alpha 1, COL3A1, Ehlers-Danlos Syndrome Type IV Autosomal Dominant, Fetal Collagen, MGC93704, Type III Collagen) (HRP)

Size

£882.00

SKU:: C7510-44E-HRP
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Purified collagen III from rat skin.
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Extra Details:: Applications: Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry (frozen and paraffin), and RIA. Other applications not tested. Recommended Dilution: immunohistochemistry (Frozen): 1:250-1:1000 immunohistochemistry (Paraffin): 1:250-1:1000 ELISA: 1:100-1:400 Immunofluorescence: 1:80 Radioimmunoassays: 1:25-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.