Collagen Type II, Rat Sternal Cartilage

Size

£557.00

SKU:: C7510-32
Molecular Weight:: 300
Purity:: >99% (SDS-PAGE), free of pepsin and proteoglycans
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 0.05M acetic acid.
Extra Details:: Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched. Highly purified type II collagen. Used for assaying antibodies in sera from collagen-induced arthritis (CIA) models and humans by ELISA. Polymeric or fibrilar collagen can bind, nonspecifically, to immunoglobulins in serum samples, secondary antibodies and avidin-peroxidase creating high, false-positive reactions. To prevent this, and to obtain consistent assay results, USBio provides highly purified, polymeric-free, ELISA Grade Collagen. Form: Solution in 0.05M acetic acid Source: Rat sternal cartilage Molecular Weight: 300kD Purity: ≥ 99% by SDS-PAGE, Free of pepsin and proteoglycans Dilution Buffer: C7510-32A Collagen-Dilution Buffer Dilution: It is recommended to dilute this product to 5-10ug/ml on ice with chilled C7510-32A Collagen-Dilution Buffer to coat ELISA plates just before use. For coating, use aliquots (do not keep diluted solutions). The optimum concentration of collagen to coat ELISA plates varies upon the type of plate used. Storage: Store at -20°C in the dark. This product is stable at 4°C for 1 week, and at -20°C for 1 year as supplied, but will gradually form polymeric collagen and degrade under neutral conditions.

Shipping Conditions:: Blue Ice