Collagen Type II

Size

£540.00

SKU:: C7510-21C
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Collagen Type II from human knee cartilage and bovine nasal cartilage.
Format:: Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Extra Details:: Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched. Applications: Suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation, Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:5000-1:50,000 Immunohistochemistry: 1:50-1:400. Antigen retrieval with sodium citrate buffer, pH 6.0 is recommended. Immunoprecipitation: 1:100 Western Blot: 1:1000-1:10,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice