C7510-19AX-FITC
Collagen Type II (BSA & Azide Free) (FITC)
Size
£1,183.00
- SKU:
- C7510-19AX-FITC
- Purity:
- Purified by Protein G affinity chromatography from ascites
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified type II collagen obtained from the adult chicken sternum by limited pepsinization. Epitope: Triple helix of type II collagen. Cellular Localization: Cartilage matrix
- Clone:
- 0.N.226
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- Applications: Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Formalin/paraffin): Staining of formalin/paraffin tissues requires digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 minutes at RT or 10 minutes at 37°C. Optimal dilutions to be determined by the researcher. Positive Control: Cartilage in lung or fetus Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice