C7510-18Z
Collagen Type II
Size
£1,010.00
- SKU:
- C7510-18Z
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- A purified preparation of lathyritic type II collagen from embryonic chicken sternum. Epitope: Carboxyl-terminal one quarter of the type II collagen, not susceptible to digestion of lathyritic type II collagen with recombinant stromelysin. Cellular Localization: Cartilage matrix.
- Clone:
- 3F134 (2B1.5)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
- Extra Details:
- Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched. Applications: Suitable for use in Flow Cytometry, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1-2ug/ml for 2 hours at RT. Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT. Requires digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 minutes at RT or 10 minutes at 37°C. Optimal dilutions to be determined by the researcher. Positive Control: Cartilage in lung or fetus. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice