C7510-17U-HRP
Collagen Type I (HRP)
Size
£927.00
- SKU:
- C7510-17U-HRP
- Purity:
- Purified by ammonium sulfate precipitation and chromatography on DEAE-cellulose from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human collagen type I
- Clone:
- 2Q652 (C11)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- Applications: Suitable for use in ELISA, Immunohistochemistry and RIA. Not suitable for Western Blot. Other applications have not been tested. Recommended Dilution: ELISA: Use as capture or as a second detecting antibody in sandwich format (Apparent binding constant is ~0.1nM). Immunohistochemistry: Acetone or ethanol fixed frozen 10um sections. Staining is similar to that for polyclonal antibodies to interstitial collagen I. Not suitable for paraffin-embedded sections or formaldhyde fixation. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice