C7510-17U-APC

Collagen Type I (APC)

Size

£927.00

SKU:: C7510-17U-APC
Purity:: Purified by ammonium sulfate precipitation and chromatography on DEAE-cellulose from ascites.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human collagen type I
Clone:: 2Q652 (C11)
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Extra Details:: Applications: Suitable for use in FLISA, Immunohistochemistry and RIA. Not suitable for Western Blot. Other applications have not been tested. Recommended Dilution: ELISA: Use as capture or as a second detecting antibody in sandwich format (Apparent binding constant is ~0.1nM). Immunohistochemistry: Acetone or ethanol fixed frozen 10um sections. Staining is similar to that for polyclonal antibodies to interstitial collagen I. Not suitable for paraffin-embedded sections or formaldhyde fixation. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.