Collagen Type I (HRP)

Size

£1,215.00

SKU:: C7510-17K-HRP
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Human placental collagen type I isolated by limited pepsin digestion and selective salt precipitation.
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish peroxidase (HRP).
Extra Details:: Type I collagen is the most common of the collagens in vertebrates. It comprises up to 90% of the skeletons of the mammals and is also widespread all over the body. Such as: skin, tendons, ligaments, cornea, intervertebral disks, dentine, arteries and granulation tissues as the main locations. The importance of type I collagen for medical research is that it is involved in many human and animal diseases, including fibrosis, osteoporosis, cancer, atherosclerosis etc. Type I collagen is frequently utilized to monitor physiological changes in tissues as well as being used as diagnostic tools in various pathological conditions. Applications: Suitable for use in Dot and Slot Blot, ELISA, Indirect Immunohistochemistry and Indirect Immunocytochemistry. Not recommended for paraffin tissue sections. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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