Collagen Type I

Size

£540.00

SKU:: C7510-11C
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Collagen Type I from human and bovine placenta
Format:: Supplied as a liquid in PBS, pH 7.2 with 0.01% sodium azide. No stabilizing proteins added.
Extra Details:: Collagens are highly conserved throughout evolution and are characterized by a n uninterrupted “Glycine-X-Y” triplet repeat that is a necessary part of the triple helical structure. It is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on non-denatured three-dimentional epitopes. Collagens from human and bovine placenta and cartilage were extensively purified by limited pepsin digestion and selective salt precipitation. This preparation resulted in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to product “type” specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducting conditions of SDS-PAGE and Immunoblot are used. Applications: Suitable for use in ELISA, Immunoprecipitation, native PAGE, Immunohistochemistry and Western Blot Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:10,000 ELISA: 1:5000-1:50,000 Immunohistochemistry: 1:50-1:200 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice