C7507-20F

Colchicum autumnale (CA) (FITC)

Size

£457.00

SKU:: C7507-20F
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 0.01M phosphate, 0.15M sodium chloride, pH 7.3, 0.05% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:: Pure Colchicum autumnale lectin (CA) Meadow Saffron, FITC conjugated. Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose. Carbohydrate Specificity: Not yet determined. Inhibitory Carbohydrate: Lactose > N-Acetylgalactosamine > Galactose Activity: Less than 0.5ug/ml will agglutinate human type A2 erythrocytes. FITC/Protein Ratio (OD495/OD280): 0.87 Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 6 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.