Cofilin 2 (CFL2)

Size

£927.00

SKU:: C7506-51
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa1-10 (MA[pS]GVTVNDE-C) of human phospho-Cofilin 2, with a C-terminal cysteine added for conjugation purposes, conjugated to KLH. Species Sequence Homology: chicken, mouse; 100%.
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Extra Details:: Cofilin is a small actin-binding protein that plays a pivotal role in cytokinesis, endocytosis, embryonic development, stress response and tissue regeneration. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1-4ug/ml detects phospho-Cofilin 2 in RIPA lysates from L6 cells, treated with LIM Kinase 1, active, Recombinant for 30 minutes. Cofilin 2 is phosphorylated at Ser3 by LIM Kinase. 500ug L6 lysate was incubated with or without 100mU LIM Kinase at 30°C for 30 minutes. Phosphorylation was terminated by boiling in reducing sample buffer. Aliquots (10ug) were resolved by electrophoresis and subjected to Western Blot, using 1ug/ml C7506-51. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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