C6010-05

CNG4 (Cyclic Nucleotide-Gated Channels 4, b-Subunit)

Size

£754.00

SKU:: C6010-05
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A 24aa synthetic peptide sequence near C-terminus of rat CNG4. (KLH).
Format:: Supplied as a liquid in PBS, pH 7.4 0.1% BSA.
Extra Details:: The cyclic nucleotides cAMP and cGMP are implicated in signal transduction events such as the visual transduction, relaxation of smooth muscles, intestinal secretion of water and salt, reabsorption of Na+ and water in the distal tubule of the nephrons. cAMP/cGMP activate Ca2+ -permeable ion channels called cyclic nucleotide-gated channels (CNG or CNC). Activation of CNG leads to depolarization of the membrane voltage and to a concomitant increase of the cytosolic Ca2+. CNG consists of two distinct subunits, designated a and b subunits. Several CNG a-subunits (CNG alpha 1-3) & beta subunits (subunit 2 or CNGb1-2 or CNG4-5) and numerous isoforms. a-subunit can form functional channel by themselves, whereas, b-subunits modulate the channel property of a-subunits. CNG display intracellular N and C-termini, 6 transmembrane domains or segments (S1-S6). The region between S5 and S6 contains the ion-conducting pore (P). The cyclic nucleotide-binding region is found at the c-terminus. Native functional CNG may exists as heteromultimer containing some combination of a, and b subunits. CNG beta-subunit or subunit 2 (also called CNG4) have been cloned from the rod outer segments (hRCNC2a and hRCNC2b) and from olfactory neurons (rOCNC2, also called CNG5). Human RCNC2a (623 aa; missing 1-286 aa) and RCNC2b (909 aa) are alternatively splice and only differ in their N-terminus. The two b proteins show ~30% identity with human a CNG1. Additional alternatively spliced CNG4D (missing 522-530 aa) and CNG4E (missing 515-532 aa) are also expressed. CNG b-subunits form heterooligomeric complex with CNG1-3. The olfactory b-subunit is expressed throughout the nasal epithelium, especially in olfactory sensory neurons, and in the vomeronasal organ. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1-10ug/ml (ECL). ELISA: 0.5-1ug/ml C6010-08 can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice